One-step labelling of oligonucleotides with fluoresceine during automated synthesis
نویسندگان
چکیده
منابع مشابه
One-step labelling of oligonucleotides with fluoresceine during automated synthesis.
Fluorescent or biotin labelling of an oligonucleotide at it's 5'-end usually involves two steps. First, a N-protected aminoalkyl phosphoramidite derivative (as for instance Aminolink 2 from Applied Biosystems) is added to the 5'-end of an oligonucleotide during automated DNA synthesis. After removal of all protecting groups the NHS ester of an appropriate fluorescent dye or biotin is coupled to...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1990
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/18.11.3427